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Titration of labelled hepcidin into serum samples. Labelled hepcidin was spiked into sera with low (a), medium (b) and high (c) levels of endogenous hepcidin (estimated from SELDI spectra). The endogenous hepcidin produces a peak at m/z 2791 and the labelled hepcidin a peak at m/z 2801. Labelled hepcidin concentrations are: bold red line: 0 ng/ml, dashed blue line: 100 ng/ml, dashed green line (200 ng/ml), dashed black line: 400 ng/ml.
