Figure 2.

Rho activity contributes to the increased contractility of Ras-transformed MCF10A cells. Ras-transformed MCF10A cells were cultured on silicone rubber substrates at high density. A–D are images of successive time points from a video recording of cells microinjected with the Rho-inhibitor C3. Cells before injection are seen in A. B shows the cells at the time of injection. Cells were injected with C3 on the right side of the field and are indicated with arrowheads. Within 4.5 min after injection (C), many of the wrinkles in the rubber have decreased. This decrease in wrinkling is more pronounced at about 9 min after injection (D).