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. Author manuscript; available in PMC: 2008 Oct 22.
Published in final edited form as: Vaccine. 2006 Nov 10;25(8):1534–1543. doi: 10.1016/j.vaccine.2006.10.036

Figure 4.

Figure 4

Cytokine gene expression in PBMC from asymptomatic L. infantum infected dogs and healthy control dogs (Group 2) after stimulation with P-8 antigen from L. pifanoi. QRT-PCR data were analysed by using the 2−ΔΔCt method. Differences in gene transcription after stimulation are expressed as n-fold difference relative to the calibrator (unstimulated cells). Shown are the cytokine expression (IFN-γ; TNF-α; IL-10; IL-18) for each infected animal (V-25, V-38 and V-27) as well as controls (C-1, C-2). Grey bars represent results from P-8 stimulation, black bars represent SLA stimulation and open bars represent ConA stimulation. Standard deviations are indicated and range from 0.0001 to 0.300.