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Adenylyl cyclase activity in OptiPrep gradient fractions prepared from fibroblasts. Plasma membranes were fractionated similarly to those shown in Figure 5. (A) Protein content of each 1-ml fraction. (B) cAMP produced in an assay tube containing 120 μl of gradient fraction incubated with 25 μM forskolin. (C) Specific activity of forskolin-stimulated adenylyl cyclase.
