Table 2.
Quantification of caveolin and Giα immunogold in caveolae-enriched and caveolae-poor areas of fibroblast plasma membranes
| Antibodies | Caveolae-enriched
areaa
|
Caveolae-poor
areab
|
||
|---|---|---|---|---|
| Caveolae/μm2 | Gold/μm2 | Caveolae/μm2 | Gold/μm2 | |
| Caveolin | 9.1–11 | 78–83 | 0–0.7 | 3.6–7.1 |
| Giα | ||||
| B087 | 8.5–9.2 | 4.2–5.4 | 0.2–0.4 | 34–41 |
| A569 | 8.7–9.7 | 9.9–15 | 0.0–0 | 31–38 |
The quantification is presented as the range of the average numbers obtained by two investigators who independently chose multiple square micrometer areas to count all invaginated caveolae and gold particles present. Each investigator counted 8 μm2 for caveolin antibodies (from three micrographs). Fifteen square micrometers were counted for αi antibodies, B087 (from eight micrographs) and A569 (from seven micrographs).
a
Caveolae-enriched areas have ≥ 5 invaginated caveolae per square micrometer.
b
Caveolae-poor areas have ≤ 2 invaginated caveolae per square micrometer.