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. 1997 Dec;8(12):2379–2390. doi: 10.1091/mbc.8.12.2379

Figure 6.

Figure 6

RanGTP modulates the interaction of p97 with RanBP2 and p62. (A) Effect of Ran on the binding of p97 to RanBP2. RanBP2-coated microtiter wells were incubated with 10 nM His-p97 and with an increasing amount of RanGTP or RanGDP. After incubation and washing, proteins were cross-linked, and the amount of bound p97 was quantified using antibodies (see MATERIALS AND METHODS). (B) Effect of Ran on the binding of p97 to p62. The analysis was carried out as in panel A, except that p62-coated microtiter wells were used.