Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Purification of RanBP2
| Step | Proteins (μg) | RanBP2 (μg) | Fold-purification
|
Yield (%) | |
|---|---|---|---|---|---|
| Step | Cumulative | ||||
| Starting material (NE) | 22,000 | 50 | 100 | ||
| Salt wash | 15,000 | 48 | 1.4 | 1.4 | 98 |
| Solubilization | 10,000 | 37 | 1.1 | 1.6 | 74 |
| Ran affinity | 200 | 17 | 23 | 37 | 34 |
| Mono Q | 2.5 | 2.5 | 11.7 | 440 | 5 |
