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The amounts of nuclear and cellular Oct-1 protein during cellular aging and immortalization. (A) The Oct-1-DNA complexes were detected in EMSA with the nuclear extracts of HuS-L12 at indicated PDLs and IML12–4 (im). (B) The amounts of nuclear Oct-1 protein were determined in the same nuclear extracts as panel A by Western blotting. (C) The total amounts of cellular Oct-1 protein were determined in the whole cell extracts of HuS-L12 at indicated PDLs and IML12–4.
