Figure 5.
SET1 strains have shortened telomeres. Genomic DNA was prepared and analyzed by XhoI digestion and genomic blotting, probed with a 350-bp telomere-specific probe that contains C1–3A telomeric repeats and a portion of the Y′ subtelomeric repeat. Telomeric DNA was compared for two independent cultures of SET1 (UCC1001) and set1 (LPY1297) cells. Telomeric repeat DNA for both set1 isolates is approximately 50 bp shorter than SET1 strains (arrowhead). Analysis of multiple independent set1 isolates reveals that the decrease in telomeric repeat length is a consistent feature of the mutant strains.