Figure 5.

ADP ribosylation of RhoA by DC3B (48 h) inhibits GTPγS (50 μM)-induced RhoA translocation from the cytosolic to the membrane fraction. (A) After incubation with or without DC3B (48 h), α-toxin–permeabilized tissues were stimulated with GTPγS (50 μM) for 20 min and homogenized and fractionated (see MATERIALS AND METHODS); translocation of RhoA to the membrane fraction was inhibited by DC3B treatment (48 h). Representative Western blots of RhoA. (B) Summary of the effect of DC3B on the translocation of RhoA by GTPγS (50 μM) from cytosolic to the membrane fraction. DC3B significantly inhibited translocation of RhoA.