Figure 6.

Recovery of the immunoprecipitability of cytosolic RhoA by detergent treatment. Cytosolic fractions of control (untreated) and DC3B (48 h) treated tissues were incubated without or with 0.05% SDS for 30 min on ice and immunoprecipitated with anti-RhoA-agarose conjugated monoclonal antibody overnight at 4°C. Samples were electrophoresed and transferred to PVDF membranes. Membranes were blotted with anti-RhoA monoclonal antibody and visualized by enhanced chemiluminescence. WE, whole extract (before immunoprecipitation); S, supernatant (after immunoprecipitation); IP, immunoprecipitate. Note that the immunoprecipitating antibody heavy (H) and light (L) chains were recognized by the blotting antibody.