Figure 7.

Cytosolic RhoA coimmunoprecipitates with rhoGDI in the absence of detergent. Cytosolic fractions of control (untreated) and DC3B-treated tissues were incubated without or with 0.05% SDS for 30 min on ice and immunoprecipitated with anti-rhoGDI polyclonal antibody overnight at 4°C and subsequently incubated with protein A-agarose for 1 h at room temperature. Samples were electrophoresed and transferred to PVDF membranes. Membranes were blotted with anti-RhoA monoclonal antibody (panel A) or antirhoGDI polyclonal antibody (panel B) and visualized by enhanced chemiluminescence. WE, whole extract (before immunoprecipitation); S, supernatant (after immunoprecipitation); IP, immunoprecipitate. In the absence, but not in the presence, of 0.05% SDS, cytosolic RhoA coimmunoprecipitates with rhoGDI.