Figure 6.

Effect of soluble VN and FGF-2 on GM 7373 cell adhesion. (A) GM 7373 cells were incubated in suspension for 90 min at 37°C with increasing concentrations of VN (O) or FN (•). Then, cells were centrifuged to remove unbound molecules and seeded onto non-tissue culture plates coated with 10 μg/ml of FGF-2 and saturated with 10 mg/ml BSA. (B) In a parallel experiment cells were incubated for 90 min at 37°C with increasing concentrations of FGF-2, centrifuged, and seeded onto non-tissue culture plates coated with 10 μg/ml of VN (O) or FN (•) and saturated with 10 mg/ml BSA. In both experiments, the number of adherent cells was evaluated after 2 h of incubation at 37°C, corrected for the nonspecific cell adhesion measured onto BSA-coated wells, and expressed as percentage of cells adherent to the different substrata when preincubated the in absence of soluble molecules. Each point is the mean of three determinations in duplicate. SEM does not exceed 13% of the values.