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. 2008 Oct 27;205(11):2483–2490. doi: 10.1084/jem.20080039

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© 2008 Hammerschmidt et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.jem.org/misc/terms.shtml). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).

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Figure 2. — Heterotopic chimeric Tx-pLN, but not orthotopic Tx-mLN, fail to generate gut-homing T cells in vivo. Cells isolated from OT-I or OT-II Ly5.1 mice were labeled with CFSE and adoptively transferred into mice that received Tx-pLN or Tx-mLN 8 wk before. 1 d later, a single dose of Ova was applied orally or injected s.c. (A) Representative results obtained for α4β7-integrin and CCR9 expression by OT-I T cells (DAPI⁻Vα2⁺Vβ5⁺CD8⁺) activated in the mLN, Tx-mLN, and Tx-pLN after oral antigen application and in pLN after s.c. injection of antigen. (B and C) Diagrams depict expression of α4β7-integrin and CCR9 as fold isotype control for OT-I T cells (DAPI⁻Vα2⁺Vβ5⁺CD8⁺; B) and OT-II T cells (DAPI⁻Ly5.1⁺Vβ5⁺CD4⁺; C). All experiments have been performed at least three times with two or more mice per group. Error bars represent SD.