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. 2008 Oct 27;205(11):2515–2523. doi: 10.1084/jem.20080829

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© 2008 Koch et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.jem.org/misc/terms.shtml). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).

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Figure 5. — Normal T cell development and absence of immature thymic B cells in BM chimeras reconstituted with DL4^ΔMx BM. Ctrl or DL4^ΔMx CD45.2⁺ BM was transplanted into lethally irradiated CD45.1⁺ hosts and analyzed 8 wk after reconstitution. The reconstitution efficiency for both Ctrl and DL4^ΔMx BM chimeras was >95% (not depicted). (A) Representative flow cytometric analysis of CD45.2⁺ thymocytes stained with anti-CD4 and -CD8. (B) CD45.2⁺ lineage-negative DN thymocytes were analyzed for the expression of CD44 and CD25. (C and D) Lineage-negative thymocytes were analyzed for the presence of B cells expressing B220 and IgM, or BP1 and CD93. Data in A–D are representative of three individual chimeras per group with virtually identical results, and relative percentages are indicated in the contour plots. Two independent experiments were performed. (E) Southern blot analysis of ScaI-digested genomic DNA derived from BM cells of Ctrl and DL4^ΔMx chimeras showing the floxed alleles in Ctrl chimeras and the completely deleted DL4 locus in DL4^ΔMx chimeras. The targeting strategy and size of the restriction fragments are as described in Fig. 2 A.