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. 2008 Oct 27;205(11):2561–2574. doi: 10.1084/jem.20081212

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© 2008 Martín-Fontecha et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.jem.org/misc/terms.shtml). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).

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Figure 2. — CD40L⁺ CD4⁺ T_EM cells license DCs for T cell priming in vitro. (a) Proliferation of CFSE-labeled HA-specific 6.5 transgenic naive CD4⁺ T cells cultured with 50 μM HA_110-119–pulsed syngeneic immature DCs in the absence or presence of ex vivo–isolated OVA-specific DO11.10 transgenic CD4⁺ T naive, T_CM, or T_EM cells. (b) Proliferation of CFSE-labeled OVA-specific OT-II transgenic naive CD4⁺ T cells cultured with 0.1 μM OVA_323-339–pulsed syngeneic immature DCs in the absence or presence of ex vivo–isolated polyclonal T naive, T_CM, or T_EM cells. (c) Proliferation of CFSE-labeled OVA-specific OT-II transgenic naive CD4⁺ T cells cultured with polyclonal T_EM cells in the presence of OVA-pulsed wild-type or CD40^−/− immature DCs. Shown is the CFSE profile of T cells measured on day 5. Results are representative of at least three independent experiments.