Figure 6.
EAE can be induced by presentation of MOG peptides in reactive lymph nodes and is inhibited by CD62P blockade. Groups of mice (n = 16), either untreated (a and b) or that had received 30 d earlier an s.c. injection of CFA or two consecutive s.c. injections of LPS-matured DCs to induce a chronic reactive lymph node (c and d), were challenged i.v. with 100 μg MOG35-55 peptide in CFA (a) or in PBS (b–d). For each experimental group, half of the mice received once a week an i.v. injection of 100 μg CD62P antibodies (circles) or isotype matched control antibodies (squares) for the entire duration of the experiment. Mice were graded daily for clinical manifestation of EAE. Shown are mean clinical scores of eight mice per group. Error bars represent the SE for each point. (right) CD4 expression on lymphocytes isolated from the brain and spinal cords of mice 20 d after priming with MOG peptide. Numbers are the percentage of the gated population. Results are representative of two separate experiments.