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. 2008 Oct 27;205(11):2609–2621. doi: 10.1084/jem.20081370

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© Cavassani et al.

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Figure 7. — TLR3 was required for chemokine generation by WT peritoneal macrophages after co-culture with necrotic neutrophils. (A) The specificity of polyclonal rabbit anti-TLR3 was determined by Western blot analysis of TLR3 expression in the following samples: lane 1, WT macrophages; lane 2, tlr7^−/− macrophages; lane 3, tlr3^−/− macrophages; and lane 4, recombinant TLR3. The black line indicates that intervening lanes have been spliced out. (B-E) Chemokine concentrations in the supernatants of WT peritoneal macrophages co-cultured with necrotic neutrophils with IgG or anti-TLR3 polyclonal antibody were quantified using Bio-Plex. The data are means ± SEM of triplicate wells and are representative of three independent experiments. *, P < 0.05; **, P < 0.01; and ***, P < 0.001 compared with IgG group.