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. 2008 Oct 27;205(11):2657–2671. doi: 10.1084/jem.20072316

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© 2008 Zhou et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.jem.org/misc/terms.shtml). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).

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Figure 4. — Lentiviral-mediated suppression of AHI-1 expression causes reduced proliferative activity and GF independence of BCR-ABL–transduced human CB cells. (A) Q-RT-PCR analysis of the levels of human AHI-1 transcripts relative to GAPDH in transduced lin⁻CD34⁺ CB cells, including cells transduced with a control vector (Ctrl), AHI/sh4 cells (with suppression of AHI-1), BCR-ABL (B/A), and AHI/sh4 cotransduced cells (B/A + AHI/sh4). (B) Growth of each transduced population in suspension cultures ± GF for 12 d. Viable cell numbers were determined by hematocytometer counts of trypan blue-excluding cells. (C) Number of BFU-E, CFU-GM, and CFU-GEMM colonies produced in semisolid cultures from the same cells as shown in B. Values shown are the mean ± SEM of triplicate measurements. * indicates significant difference between BCR-ABL–transduced cells alone and cells cotransduced with BCR-ABL and AHI/sh4.