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. 2008 Oct 20;14:1856–1864.

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This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Localization of GFP-NHS-A400delC mutant in MDCK cells. Cells were transfected with GFP-NHS-A and GFP-NHS-A400delC fusion constructs and pEGFP-C1 control. Transiently expressed fusion proteins were visualized by confocal microscopy. GFP-NHS-A wild type protein primarily localized to the cellular periphery whereas GFP-NHS-A400delC mutant protein localized in the cytoplasm and nucleus. Apparent peripheral distribution of GFP is an experimental artifact seen only between some adjoining transfected cells. Images were taken with a 60X objective.