Fig. 3.

Detection of Leishmania infantum DNA using the FTA method. (A) Serial dilutions of cultured L. infantum were spotted onto FTA paper, then extraction and PCR performed using primers specific for the parasite small subunit ribosomal RNA. Numbers above bands represent the number of parasites spotted onto the FTA paper. (B) Detection of L. infantum in lab-infected Lu. longipalpis submitted to whole fly FTA extractions from 24 h to 120 h post-infection. Negative control (C−) represents FTA extraction from an uninfected sand fly, and positive control (C+) represents genomic Leishmania DNA extracted from an infected sand fly amplified using the same set of primers.