TABLE IV.

Parameters from Boltzmann Fits to G-V Relations

Channel	V1/2	ΔV1/2	z	Δz	n
Ctrl	−30.3 ± 0.9	n/a	2.4 ± 0.2	n/a	3–11
S654C	−33.3 ± 0.6a	−2.0	2.3 ± 0.12	−0.11	7
I655C	−30.9 ± 0.9	−0.6	1.82 ± 0.11a	−0.59	11–12
F656C	−28.0 ± 0.6	+2.3	1.99 ± 0.09	−0.41	5
G657Cb	−67.6 ± 0.5c	−37.3	2.70 ± 0.24	−0.30	5
N658C	−3.38 ± 0.9c	+26.9	1.58 ± 0.09a	−0.83	3
V659C	−23.8 ± 1.9a	+6.5	1.11 ± 0.10a	−1.30	3
S660C	−27.6 ± 1.0	+2.7	1.99 ± 0.15	−0.41	4
A661C	−8.6 ± 0.5c	+21.7	1.97 ± 0.07a	−0.43	7
I662C	−44.5 ± 1.5c	−14.2	1.70 ± 0.15a	−0.71	11
I663C	−7.7 ± 0.6c	+22.6	1.94 ± 0.08a	−0.46	9
Q664C	−22.8 ± 0.5a	+7.5	2.38 ± 0.11	−0.02	3–6
R665Cb	−30.5 ± 0.4	−0.20	2.9 ± 0.16a	+0.07	4
L666C	−32.2 ± 0.8	−1.9	2.46 ± 0.16	+0.05	3–11
Y667C	−36.9 ± 0.4a	−6.6	2.47 ± 0.08	+0.06	3
S668Cb	−44.4 ± 1.7c	−14.1	2.93 ± 0.58	+0.53	15

Values are derived from Boltzmann fits to steady-state activation data (see Materials and methods). Values are ± SEM.

^aP < 0.05.

^bFit with an empirical double Boltzmann function (see Materials and methods). Values corresponding to the first function are reported.

^cP < 0.001.