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. 2008 Nov;132(5):547–562. doi: 10.1085/jgp.200810051

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© 2008 Jara-Oseguera et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.jgp.org/misc/terms.shtml). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).

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Figure 8. — Channel-closing kinetics in the presence of TEA. (A) Representative tail currents obtained at −180 mV after a prepulse of 100 ms at 60 mV in the absence (thick trace) or presence of 20, 40, and 80 mM TEA (gray traces). Dotted lines are fits to a single exponential function. (B) Channel-closing rate as a function of voltage obtained from fits to an exponential as in A. The straight lines are fits to the equation:
Symbols and parameters of the fit are (n = 4): No TEA k₁(0) = 196.49 s⁻¹, z₁ = 0.073 e_o (filled circles); 20 mM TEA k₁(0) = 364.9 s⁻¹, z₁ = 0.04 e_o (filled triangles); 40 mM TEA k₁(0) = 323.9 s⁻¹, z₁ = 0.083 e_o (filled diamonds); 80 mM TEA k₁(0) = 457.2 s⁻¹, z₁ = 0.086 e_o (filled squares). The closing rate increases with blocker concentration, indicating that TEA speeds up channel closure. (C) Representative traces of single-channel openings in the absence or the presence of the indicated concentration of TEA. The dotted lines indicate the current amplitude in the absence of TEA. (D) Burst length distributions obtained from traces as in C. Burst length was measured and compiled in logarithmically binned histograms in the absence or presence of TEA. The black lines are fits with a single exponential function of time with the following time constants: No TEA, 3.17 ms; 2.5 mM TEA, 1.2 ms; 5 mM TEA, 0.8 ms.

graphic file with name M8.gif — Channel-closing kinetics in the presence of TEA. (A) Representative tail currents obtained at −180 mV after a prepulse of 100 ms at 60 mV in the absence (thick trace) or presence of 20, 40, and 80 mM TEA (gray traces). Dotted lines are fits to a single exponential function. (B) Channel-closing rate as a function of voltage obtained from fits to an exponential as in A. The straight lines are fits to the equation:
Symbols and parameters of the fit are (n = 4): No TEA k₁(0) = 196.49 s⁻¹, z₁ = 0.073 e_o (filled circles); 20 mM TEA k₁(0) = 364.9 s⁻¹, z₁ = 0.04 e_o (filled triangles); 40 mM TEA k₁(0) = 323.9 s⁻¹, z₁ = 0.083 e_o (filled diamonds); 80 mM TEA k₁(0) = 457.2 s⁻¹, z₁ = 0.086 e_o (filled squares). The closing rate increases with blocker concentration, indicating that TEA speeds up channel closure. (C) Representative traces of single-channel openings in the absence or the presence of the indicated concentration of TEA. The dotted lines indicate the current amplitude in the absence of TEA. (D) Burst length distributions obtained from traces as in C. Burst length was measured and compiled in logarithmically binned histograms in the absence or presence of TEA. The black lines are fits with a single exponential function of time with the following time constants: No TEA, 3.17 ms; 2.5 mM TEA, 1.2 ms; 5 mM TEA, 0.8 ms.