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. 2008 Oct 31;3(10):e3601. doi: 10.1371/journal.pone.0003601

Figure 4. Impairment of NK-triggered Th1 polarization by DCs following HIV-1 infection is associated to altered IL-12 and IL-18 production.

Figure 4

(a) Th1 polarization by DCs triggered by NK cells was tested by incubating iDC (106/ml) for 30 mn in the presence of rNK or aNK cells (2×105/ml). Naïve CD4 T cells (106/ml) were added to the cocultures and the frequency of T cells producing IFN-γ or IL-4 was determined by flow cytometry 8 days later. The experiment was performed with either uninfected iDCs (b) iDCs infected with HIV-1BaL (c), or iDC infected with HIV-1BaL in the presence of AZT (1 mM) (d). Culture supernatants of indicated cultures were tested for IL-12 (e), IL-18 (f), and IFN-g (g) content. Data represent the mean±sd of five independent experiments. Statistical comparisons were made with the non-parametric Mann-Whitney test. * p<0.05, **p = 0.03.