Figure 2.

Effect of HLA-A2 on the initial rate of IR autophosphorylation in liposomes. Rates are plotted as log[P_I- P_t/P_I] versus time, where P_I is level of maximal phosphorylation and P_t is level of phosphorylation at time t. ³²P-ATP-containing liposomes were made with or without 10:1 HLA-A2/IR. Phosphorylation was stimulated by adding insulin to a final concentration of 1 nm. ○, IR alone; ▪, HLA-A2/IR 10:1. Solid and dashed lines are best fits to the data. For IR alone y = −0.01 to 0.029x; for HLA/IR y = −0.11 to 0.075x. Correlation coefficients are 0.99 for IR alone and 0.98 for HLA/IR.