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. 1997 Dec;8(12):2463–2474. doi: 10.1091/mbc.8.12.2463

Figure 4.

Figure 4

(A) Insulin-stimulated autophosphorylation of the β subunit of IR in B-LCL cells. Cells were metabolically labeled with H332PO4 followed by treatment with 10−9 M insulin at 37°C as described in MATERIALS AND METHODS, IR was immunoprecipitated from cell extracts by anti-IR b chain antibody. Each value is the average of three experiments. (B) Autophosphorylation as a function of insulin concentration in 721.1 (HLA:IR 1.5:1) and 961 (HLA:IR 5:1) B-LCL cells. (C) β 2m and autophosphorylation of IR in the B-LCL line 721.13 (HLA/IR 15/1). Cells were preincubated with 6 μm β2m overnight followed by treatment with 10−9 M insulin.