Fig. 3.

Quantitative real-time RT–PCR analysis of promoter-specific CIITA transcripts in various cells. THP1-L cells were treated with 5-aza for 5 days and then either left untreated or incubated with IFN-γ for 2 days. THP1-pc, untreated or similarly incubated with IFN-γ, iDCs and the B cell Raji were also analysed. For AIR-1 p-I, the housekeeping normalized PCR data value obtained in iDC was defined as the arbitrary unit (value 1). For AIR-1 p-III, the housekeeping normalized PCR data value obtained in Raji was defined as the arbitrary unit (value 1). For AIR-1 p-IV, the housekeeping normalized PCR data value obtained in THP1-pc treated with IFN-γ was defined as the arbitrary unit (value 1). The results shown are from one representative experiment of two with similar results.