Table 3.
Specific activities and kinetic parameters for the pentose phosphate pathway in Zymomonas mobilis.
| Enzyme | Symbol in the Model | Specific Activity(†) | Kinetics Parameters | Comments | References |
|---|---|---|---|---|---|
| Xylose Uptake (Upt) | X8 | 29509 U/mg | [36] | ||
| Km = 92-148 mM (Xylose) | Glucose facilitator (glf) | [45] | |||
|
Km = 40mM (Xylose)
Vmax = 115 nmol min-1 mg[dry wt]-1 (*) |
[46] | ||||
| Transketolase-a(α) (PPP2) | X9 | 120 U/mg | [36] | ||
| 9.7 U/mg |
Km = 160 μM (Xy5P)
Km = 1400 μM (Rib5P) |
[47]. Tables 2 and 5 | |||
| Transketolase-(PPP3) | X10 | 34.29 U/mg | Km = 9950 μM (S7P)
Km = 8690 μM (GAP) |
[36] | |
| 2.7 U/mg | At 29 hr. Xylose as carbon source | [38]. Table 10 | |||
| Transketolase-b(α) (PPP4) | X11 | 120 U/mg |
Km = 160 μM (Xy5P)
Km = 90 μM (E4P) |
[36] | |
| 2.5 U/mg | At 29 h. Xylose as carbon source | [38]. Table 10 | |||
| 12.7 U/mg | Km = 90 μM (E4P)
Km = 31000 μM (Rib5P) |
E. coli | [48]. Table 1 and 2 | ||
| Km = 18 μM (E4P) | Yeast | [48]. Table 2 | |||
| Transaldolase (Gly2) | X12 | 60 U/mg | Km = 1040 μM (F6P) | [36] | |
| Glyceraldehyde-3-Phosphate Dehydrogenase (Gly3) | X13 | 25 U/mg | [36] | ||
| Km = 29 μM(GAP) | Celery leaves. No data for Z. mobilis | [49] | |||
| Phosphoglucose isomerase (α) (Gly1) | X14 | 228 U/mg | Km = 200 μM (F6P) | [36] | |
| 1.2 U/mg | At 29 h. Xylose as carbon source | [38]. Table 10 | |||
| 6-phosphogluconate dehydrogenase (PPP1) | X15 | Km = 25 μM (6PG) | [36] | ||
| 245 U/mg | Km = 40 μM (6-phosphogluconate)
Ki = 400 μM (DL-alpha-glycerophosphate) |
[50] |