Table 1.
Sequence tags obtained for those peptides observed in all analysesa
| Protein | Peptide | ECD |
CID |
||||
|---|---|---|---|---|---|---|---|
| Sequence tag |
Mass accuracy (ppm) | Sequence tag |
Mass accuracy (ppm) | ||||
| Abs | Consec | Abs | Consec | ||||
| Cytochrome c | TGPNLHGLFGR | 8.0 | 8.0 | 4.5 | 8.3 | 8.3 | 84.5 |
| Cytochrome c | EDLIAYLK | 5.0 | 4.3 | 8.1 | 4.0 | 4.0 | 125.7 |
| Alcohol dehydrogenase | VVGLSTLPEIYEKb | 8.7 | 4.3 | 4.7 | 6.0 | 4.0 | 102.3 |
| BSA | YLYEIAR | 5.0 | 4.7 | 1.7 | 5.3 | 4.7 | 147.8 |
| BSA | HLVDEPQNLIKb | 9.0 | 4.0 | 7.1 | 6.0 | 3.3 | 107.6 |
| BSA | LVNELTEFAK | 9.0 | 9.0 | 4.2 | 7.3 | 7.3 | 56.3 |
| BSA | LGEYGFQNALIVR | 8.7 | 7.0 | 3.4 | 7.0 | 5.3 | 72.8 |
| Apo-transferrin | ELPDPQESIQRb | 6.7 | 5.0 | 2.2 | 7.3 | 5.3 | 75.2 |
| Apo-transferrin | DNPQTHYYAVAVVK | 10.7 | 10.7 | 4.8 | 7.0 | 4.7 | 96.9 |
| Apo-transferrin | DKPDNFQLFQSPHGKb | 9.3 | 8.0 | 9.1 | 8.7 | 5.3 | 70.9 |
| Apo-transferrin | TYDSYLGDDYVR | 9.0 | 8.0 | 3.1 | 7.3 | 7.0 | 87.5 |
| Apo-transferrin | HSTVFDNLPNPEDRb | 10.7 | 7.0 | 4.0 | 9.0 | 7.7 | 62.4 |
| Average | 4.7 | 90.8 | |||||
a
Abs, total number (mean over three replicates) of N-Cα (ECD) or N-Co (CID) cleavages observed; Consec, number (mean over three replicates) of consecutive N-Cα (ECD) or N-Co (CID) cleavages observed. Mass accuracy of fragments is given.
b
Proline limited coverage.