Table 4.
Calculation 1 | Calculation 2 | |||||
Population | T | LDR | PR | mR | M | Total M |
WFS | 0.2 | 0.39 | 0.91 | 6.08 | 0.82 | 4,268 |
PD | 0.2 | 0.28 | 0.80 | 9.20 | 0.54 | 6,481 |
MER | 0.2 | 0.06 | 0.25 | 52.4 | 0.10 | 35,000 |
MxB | 0.2 | 0.11 | 0.45 | 25.4 | 0.20 | 7,000 |
EMAI | 0.2 | 0.58 | 0.99 | 3.42 | 1.46 | 2,397 |
WFS | 0.141 | 0.61 | 0.99 | 3 | 1.58 | 2,215 |
PD | 0.065 | 0.78 | 1.00 | 2 | 2.51 | 1,394 |
MER | 0.151 | 0.06 | 0.25 | 52 | 0.10 | 35,000 |
MxB | 0.151 | 0.28 | 0.80 | 9 | 0.54 | 6,481 |
EMAI | 0.053 | 1.00 | 1.00 | na | na | na |
In calculation 1, the threshold (T) was set to 0.2 or the empirically derived 5% significance threshold for each population. This allowed the value for LDR to be taken from the dataset and used to calculate PR. The range (R) was set to 0 – 5 cM in each case and mR = 5. In calculation 2, PR was set to 0.95 in each case and the thresholds used were the same as for calculation 1 which resulted in use of the same values for LDR. This allowed the number of markers (mR) for size range (R = 5) to be calculated. mR was converted into the required marker spacing in cM (M) and the total number of markers required for a genome scan (Total M) for each population. The calculation was not applicable (na) where LD was equal to 1.