Figure 5.

Overexpression of srw1⁺ can induce endoreplication and restores fertility to Δrum1 cells. (A) DAPI staining and (B) Flow cytometry (FACS) analysis of wild-type cells expressing srw1⁺. The srw1⁺ cDNA was deleted for G-tail, reinserted into the pREP1 vector (pREP1-srw1^+*) and transformed into wild-type cells. Cells were grown first in PM+N medium with thiamin and then washed and transferred to PM+N medium without thiamin (0 h) and incubated for the indicated times. (C) Overexpression of srw1⁺ restores fertility to Δrum1 cells. The h^- rum1::ura4⁺ ura4-D18 leu1–32 (SY100) cells transformed with pREP1-srw1^{+*, pREP1-}rum1⁺ or pREP1-X were mixed with h^+s leu1–32 (K150-A13) cells and spotted on MEA plates and incubated at 27°C for 2 d.