Table 1.
Cells lacking srw1+ are sterile but sterility is suppressed by deletion of cig2+ or inactivation of cdc2+
| Strain | % Mating
frequencies
|
|
|---|---|---|
| 27°C | 25°C | |
| h− leu1 × h+S leu1 | 57.0 | 55.2 |
| h−Δsrw1 leu1 × h+S leu1 | <0.01 | <0.01 |
| h−Δcig2 Δsrw1 leu1 × h+S leu1 | 35.6 | 34.7 |
| h+S cdc2-M35 Δsrw1 leu1 × h− leu1 | 0.44 | 0.02 |
Cells were grown to log phase in YEL, mixed and spotted on MEA plates followed by a 2-d incubation at the indicated temperature. Mating efficiencies were calculated as described in MATERIALS AND METHODS.