Table 2.

The viability of cells expressing Cdc^F15 and Cdc13

Strain	% Leu+
	pcL-X	pcL-cdc2+F15	pcL-cdc13+
h− leu1	100	41.7 ± 7.9	38.4 ± 7.6
h− Δsrw1 leu1	100	0.157 ± 0.076	20.3 ± 5.66
h− wee1-50 leu1	100	ND	48.7 ± 20.6
h+S Δsrw1 wee1-50 leu1	100	ND	1.35 ± 0.11

Cells were transfected with the indicated plasmids and selected for leu⁺ phenotype at 30°C for the h⁻ leu1-32 and h⁻ Δsrw1 leu1-32 cells and at 25°C for the h⁻ wee1-50 leu1-32 and h^+S Δsrw1 wee1-50 leu1-32 cells, both temperatures being permissive for these strains. The ratios of leu⁺ colonies formed with the indicated plamids to those formed with the empty vector are expressed as % leu⁺. Each experiment was performed three times and values of mean ± SD are shown.