Figure 3.
Somatic requirement of UNC-60A for organized assembly of actin networks in the myoepithelial sheath of the somatic gonad. rrf-1(pk1417) (RNAi-defective in soma) worms were treated with control RNAi (A-C) or unc-60A(RNAi) (D-F), and the dissected gonads stained for F-actin (A and D) and DNA (B and E). Merged images are shown in C and F (F-actin in red and DNA in blue). unc-60A(RNAi) resulted in formation of abnormal clumps of oocytes (asterisks in D and E) but did not affect actin filament organization in the myoepithelial sheath. Note that the micrographs in A-C are composites of multiple micrographs of the same gonad. Bar, 20 μm.