Table 2.
Effects of unc-60B(su158) and expression of UNC-60B variants on contractility and actin organization in the body wall muscle
Genotype | Worm motility (beating/30 sec ± s.d., n=20) | Rescue of actin organization* (%, n=50) |
---|---|---|
Wild-type | 128 ± 4.33 | NA |
unc-60B(su158) | 6.90 ± 4.00 | NA |
unc-60B(su158); GFP-UNC-60B(WT) | 99.8 ± 15.8 | 100 |
unc-60B(su158); GFP-UNC-60B(Δ150) | 1.85 ± 1.60 | 0 |
unc-60B(su158); GFP-UNC-60B(Δ152) | 72.4 ± 14.2 | 72 |
When a majority of GFP-expressing muscle cells in a worm restored striated actin organization without actin aggregates, the worm was counted as “rescued”. Wild-type and unc-60B(su158) worms with no transgene had 100 % penetrant organized and disorganized actin filaments, respectively (n>200).