Skip to main content
. 2008 Nov 7;4(11):e1000196. doi: 10.1371/journal.ppat.1000196

Figure 6. SOCS-3 mRNA transcription is induced in an NF-κB dependent manner.

Figure 6

A549 wt cells or A549 cells stably transduced with empty vector, dominant negative MKK6Ala or IKK2KD were either infected with PR8 for 3 hours (MOI = 5) (A and B) or with the influenza A virus mutant ΔNS1 and the corresponding isogenic wild type virus (G and H) [74] or transfected for 3 hours with RNA from infected or uninfected A549 cells (C–F). (E and F) A549 cells were treated with 40 µM of the NF-κB inhibitor BAY 11-7085 30 minutes prior transfection of RNA from infected (“viral RNA”) or uninfected A549 wt cells (“cellular RNA”). In all experiments shown total RNA from target cells was isolated and reverse transcribed. cDNA was subjected to quantitative real time PCR. mRNA levels of SOCS3 (A, C, E, G) or IFNβ (B, D, F, H) were assed by specific primers.