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. Author manuscript; available in PMC: 2008 Oct 23.
Published in final edited form as: Dev Dyn. 2006 Aug;235(8):2122–2133. doi: 10.1002/dvdy.20863

Fig. 10.

Fig. 10

Assessment of skeletal muscle induction of P19-SI cells by DMSO. P19-SI cells were aggregated in the presence of 0.5% DMSO. Day 4 aggregates were plated onto plastic tissue culture plates (an attachable surface), cultured for 15 days, then fixed for antibody staining (see Materials and Methods). (A) Phase contrast image of an area containing a cohort of bipolar cells. (B) The same area stained with antibody to skeletal myosin heavy chain. (C) Phase contrast image of another aggregate-derived “island”. (D) The same area stained with antibody to Pax 7 or (E) desmin. (F) Merged image of Pax 7 - and desmin-positive cells.