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. Author manuscript; available in PMC: 2008 Oct 23.
Published in final edited form as: Dev Dyn. 2006 Aug;235(8):2122–2133. doi: 10.1002/dvdy.20863

Fig. 4.

Fig. 4

BMP 4 effects on P19-SI cell cardiogenesis. P19 parental and P19-SI cells were cultured as hanging drops (50–100 cells per 30ul) as described in Materials and Methods. (□) P19-SI cells cultured with no BMP 4 added; (◆) 10ng/ml BMP 4 added at the time the hanging drop cultures were established; (▲) 10 ng/ml BMP 4 added in 3ul on day 2; (■) “parental” P19 cells cultured with 0.5% DMSO. Hanging drop cultures (32 per experimental condition) were scored daily for beating activity. BMP 4 added prior to cell aggregation (◆) inhibits cardiogenesis; BMP 4 added after the cells have aggregated (▲) enhances cardiogenesis. Control P19-SI aggregates (□) appear to “self-induce” to cardiocytes as efficiently as do “parental” P19 cell aggregates induced with DMSO (■).