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. Author manuscript; available in PMC: 2008 Oct 23.

Published in final edited form as: Dev Dyn. 2006 Aug;235(8):2122–2133. doi: 10.1002/dvdy.20863

Fig. 5 — Effect of aggregation on P19-SI cell gene expression patterns. P19-SI cells were grown in monolayer ( ) or mass aggregate ( ) cultures. Samples were harvested from 13–124 hrs and assayed by quantitative RT-PCR to detect expression of the early mesodermal marker, Brachyury T; known cardiac transcription factors (Nkx 2.5, GATA 4, Myocardin); the cardiogenic signaling molecule, BMP 4; Noggin, Wnt 3a and Msx-1. Data in b) through i) are normalized to HPRT (a) expression. Msx-1 mRNA was not measured in monolayer cultures. See text for complete discussion.

Inline graphic — Effect of aggregation on P19-SI cell gene expression patterns. P19-SI cells were grown in monolayer ( ) or mass aggregate ( ) cultures. Samples were harvested from 13–124 hrs and assayed by quantitative RT-PCR to detect expression of the early mesodermal marker, Brachyury T; known cardiac transcription factors (Nkx 2.5, GATA 4, Myocardin); the cardiogenic signaling molecule, BMP 4; Noggin, Wnt 3a and Msx-1. Data in b) through i) are normalized to HPRT (a) expression. Msx-1 mRNA was not measured in monolayer cultures. See text for complete discussion.