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. Author manuscript; available in PMC: 2008 Oct 23.

Published in final edited form as: Dev Dyn. 2006 Aug;235(8):2122–2133. doi: 10.1002/dvdy.20863

Fig. 8 — P19-SI cell cardiogenesis is inhibited by exposure to BMP 4 at the beginning of aggregation. P19-SI cultures were established in aggregation conditions either with ( ) or without ( )10ng/ml BMP4. Samples were harvested up to 110 hrs after plating and assayed by quantitative RT-PCR to detect expression of the early mesodermal marker, Brachyury T; known cardiac transcription factors (Nkx 2.5, GATA 4, Myocardin); the cardiogenic signaling molecule, BMP 4; Noggin, Wnt 3a and Msx-1. Data in b) through i) are normalized to HPRT expression. Cardiac transcription factors do not increase if BMP 4 is added before the cells have begun to aggregate. Note also the earlier increase in Msx-1 expression when BMP 4 is added at the time the aggregate cultures are established. See text for complete discussion.

Inline graphic — P19-SI cell cardiogenesis is inhibited by exposure to BMP 4 at the beginning of aggregation. P19-SI cultures were established in aggregation conditions either with ( ) or without ( )10ng/ml BMP4. Samples were harvested up to 110 hrs after plating and assayed by quantitative RT-PCR to detect expression of the early mesodermal marker, Brachyury T; known cardiac transcription factors (Nkx 2.5, GATA 4, Myocardin); the cardiogenic signaling molecule, BMP 4; Noggin, Wnt 3a and Msx-1. Data in b) through i) are normalized to HPRT expression. Cardiac transcription factors do not increase if BMP 4 is added before the cells have begun to aggregate. Note also the earlier increase in Msx-1 expression when BMP 4 is added at the time the aggregate cultures are established. See text for complete discussion.