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. Author manuscript; available in PMC: 2008 Oct 23.
Published in final edited form as: Dev Dyn. 2006 Aug;235(8):2122–2133. doi: 10.1002/dvdy.20863

Fig. 9.

Fig. 9

Analysis of markers for both cardiac and non-cardiac gene expression in P19-SI aggregates. Twenty (20) day-old beating aggregates of P19-SI cells were pooled and mRNA was prepared as described in Material and Methods. “Conventional” reverse transcription-polymerase chain reaction (RT-PCR) analysis showed that genes representative of all three germ layers are expressed in these older aggregates (lane 1). Lanes 2 & 3, showing results with mRNA isolated from control tissues, demonstrate the specificity of the primers.