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. Author manuscript; available in PMC: 2009 Jul 1.
Published in final edited form as: Mol Cancer Res. 2008 Jul;6(7):1124–1136. doi: 10.1158/1541-7786.MCR-07-2095

FIGURE 4.

FIGURE 4

Ras-mediated and TGF-β–mediated increase in VEGF expression is COX-2 independent. RIE:iRas cells were pretreated with DMSO or 10 μmol/L NS398 for 15 min and then treated with vehicle, 5 mmol/L IPTG, 3 ng/mL TGF-β, or both IPTG and TGF-β for 24 h. A. VEGF protein in conditioned medium was measured by ELISA and normalized to total protein concentration. Box plots show data from four independently replicated experiments. B. Prostacyclin (6-keto-PGF1α) levels in conditioned medium were measured by gas chromatography-mass spectrometry. Box plots show data from four independently replicated experiments. Significance between no inhibitor and NS398 treatment was determined by ANOVA with Bonferroni correction. *, P < 0.0001, compared with TGF-β; **, P = 0.09, compared with Ras; #, P < 0.0001, compared with Ras + TGF-β.