Table 1.

Multi-color BiFC Vectors

Gelvin lab stock number	Plasmid name	Autofluorescent protein fragment	Rare-cutting site flanking cassette	Protein fusiona
E3228	pSAT1-nVenus-C	nVenus	AscI	C-terminal
E3229	pSAT4-nVenus-C	nVenus	I-SceI	C-terminal
E3230	pSAT6-nVenus-C	nVenus	PI-PspI	C-terminal
E3308	pSAT1-nVenus-N	nVenus	AscI	N-terminal
E3231	pSAT1A-nVenus-N	nVenus	AscI	N-terminal
E3310	pSAT4-nVenus-N	nVenus	I-SceI	N-terminal
E3232	pSAT4A-nVenus-N	nVenus	I-SceI	N-terminal
E3233	pSAT6-nVenus-N	nVenus	PI-PspI	N-terminal
E3449	pSAT1-cCFP-N	cCFP	AscI	N-terminal
E3450	pSAT1A-cCFP-N	cCFP	AscI	N-terminal
E3451	pSAT4-cCFP-N	cCFP	I-SceI	N-terminal
E3347	pSAT4A-cCFP-N	cCFP	I-SceI	N-terminal
E3497	pSAT6-cCFP-N	cCFP	PI-PspI	N-terminal
E3307	pSAT1-nCerulean-N	nCerulean	AscI	N-terminal
E3246	pSAT1A-nCerulean-N	nCerulean	AscI	N-terminal
E3309	pSAT4-nCerulean-N	nCerulean	I-SceI	N-terminal
E3247	pSAT4A-nCerulean-N	nCerulean	I-SceI	N-terminal
E3248	pSAT6-nCerulean-N	nCerulean	PI-PspI	N-terminal
E3242	pSAT1-cCFP-C	cCFP	AscI	C-terminal
E3243	pSAT4-cCFP-C	cCFP	I-SceI	C-terminal
E3244	pSAT6-cCFP-C	cCFP	PI-PspI	C-terminal
E3415	pSAT1-nCerulean-C	nCerulean	AscI	C-terminal
E3416	pSAT4-nCerulean-C	nCerulean	I-SceI	C-terminal
E3417	pSAT6-nCerulean-C	nCerulean	PI-PspI	C-terminal
E3519	ocs-bar-RCS2-2 (T-DNA binary vector)	N/A	Multiple rare-cutting sites	N/A

^aN-vectors indicate that the protein of interest in on the N-terminus and the autofluorescent protein fragment is on the C-terminus; C-vectors indicate that the protein of interest in on the C-terminus and the autofluorescent protein fragment is on the N-terminus.

N/A, not applicable