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LC-ESFMRM/MS analysis of HεGuo and its corresponding [15N5] internal standard. (A) Authentic HεGuo standard (200 pg/mL). (B) Internal standard. (C) HεGuo (24.4 adducts per 103 normal Guo bases) from yeast tRNA (200 mg) treated with ONE (1.0 mg, 6.5 (μmol). (D). Internal standard for yeast tRNA. Ion chromatograms for HεGuo (m/z 420 → m/z 288), [15N5]HεGuo (m/z 425 → m/z 293).
