Skip to main content
. 2008 Oct 2;27(20):2712–2724. doi: 10.1038/emboj.2008.194

Figure 2.

Figure 2

Effects of Hsp70 and Hsp40 on spontaneous N, NM and Sup35 prionogenesis. (A) Unseeded, rotated (80 rpm) His–N (2.5 μM) fibrillization with ATP (5 mM) after 20 min in the presence or absence of sti, Ydj1, Sis1, Ssa1, Ssb1, Ssa1:Sis1, Ssa1:Ydj1, Ssb1:Sis1 or Ssb1:Ydj1 (2.5 μM). Fibrillization was monitored by the amount of SDS-resistant N. Values represent means±s.d. (n=3). (B) Kinetics of unseeded, rotated (80 r.p.m.) Sup35 (2.5 μM) fibrillization with ATP (5 mM) in the presence or absence of Ydj1, Sis1 (2.5–25 μM), Ssa1:Sis1, Ssa1:Ydj1, Ssb1:Zuo1:Ssz1, Ssb1:Sis1 or Ssb1:Ydj1 (2.5 μM). Fibrillization was monitored by the amount of SDS-resistant Sup35. Values represent means±s.d. (n=3). (C) His–N (2.5 μM) was assembled for 20 min, or NM (2.5 μM) or Sup35 (2.5 μM) were assembled for 6 h in the presence or absence of Ydj1, Sis1, Ssa1:Sis1, Ssa1:Ydj1, Ssb1:Zuo1:Ssz1, Ssb1:Sis1 or Ssb1:Ydj1 (2.5 μM). Reaction products were concentrated and transformed into [psi−] cells. The proportion of [PSI+] colonies was then determined. Values represent means±s.d. (n=3).