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. 2008 Oct 2;27(20):2766–2779. doi: 10.1038/emboj.2008.201

Figure 5.

Figure 5

DUX4 interferes with myogenic regulators and diminishes myotube formation. (A) qRT–PCR analyses of myogenic-specific genes in iC1C12-DUX4. DUX4 was induced with various concentrations of dox for 12 h. (B) Western blot analyses for the expression of MyoD and Myf5 in iC2C12-DUX4 over 16 h. (C) Immunofluorescence for MyoD (red) in 16 h DUX4-induced cells. Nuclei were contrastained with DAPI (blue). (D) Morphology of iC2C12-DUX4 after 4 days of differentiation in 2% horse serum. Note that DUX4-expressing cells, depending on the dox concentration, showed impaired to diminished differentiation. (E) Immunofluorescence for MyHC (red) on day 4 of differentiation of iC2C12-DUX4 cells. (F) Morphology of control (iC2C12 target) cells after 4 days of differentiation induced with 50 ng/ml doxycycline and evaluated by immunofluorescence for MyHC (G). Note that doxycycline alone does not have any significant effect on iC2C12 differentiation.