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. Author manuscript; available in PMC: 2008 Oct 24.
Published in final edited form as: J Mol Biol. 2006 Nov 3;365(5):1350–1358. doi: 10.1016/j.jmb.2006.10.102

Figure 4. Change of actin filaments mean length after 2 minutes incubation with cofilin from different sources.

Figure 4

Several snapshots of TRC~F-actin filaments being held by HMM or CapZ were taken before the addition of cofilin from different sources, and after 2 minutes incubation with cofilin at 25 °C. Images were enhanced and processed as in Fig. 2. The length of at least 150 filaments was measured in each case. The change in the filament mean length distributions was plotted vs. concentration of cofilin added to the immobilized actin filaments. 1 - yeast cofilin was added to actin filaments attached to the glass surface by HMM; 2 - yeast cofilin was added to actin filaments attached to the glass surface by CapZ; 3 - nematode cofilin was added to actin filaments attached to the glass surface by CapZ. The data obtained for S3C mouse cofilin is not shown.