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. 1997 Dec;8(12):2501–2509. doi: 10.1091/mbc.8.12.2501

Figure 3.

Figure 3

Characterization of the cdc37–34 mutant. (A) Western Blot analysis of Cdc37 protein in whole-cell extracts from cdc37–34 cells (cdc37) grown at 25°C (lane 1) or 37°C for 1 h (lane 2), cdc37–34 cells containing pRSS2 (wild-type CDC37 on a low copy number plasmid; lane 3), wild-type cells (lane 4), and cdc37–34 cells containing pGAL-37 grown in glucose (lane 5) or galactose (lane 6). Full-length Cdc37p is arrowed. Bottom panel, reprobing the same filter with antisera against phosphoglycerate kinase (PGK; arrowed). (B) Serial dilutions of yeast cells and growth at 25°C or 37°C. WT, wild-type cells; cdc37, cdc37–34; cdc37/WT, cdc37–34 with pRSS2 (low copy number plasmid with CDC37); cdc37/pGAl-37, cdc37–34 with multicopy plasmid containing CDC37 under galactose promoter control.