Figure 5.

Hormone binding to AR in wild-type and cdc37–34 mutant strains. (A) Titration of [³H]R1881 in yeast cells expressing AR. Wild-type (WT; squares) and cdc37–34 mutant cells (triangles) were tested at 25°C (open symbols) and 37°C (closed symbols). (B) Hydroxyflutamide competition assay. hsp82^G170D mutant and cdc37–34 mutant cells were incubated at 25°C (lanes 1, 2, 5, and 6, respectively) or at 37°C (lanes 3, 4, 7, and 8, respectively) with (even lanes) or without (odd lanes) 250-fold excess of hydroxyfutamide in the presence of 100 nM [³H]R1881. Results are expressed as a percentage of the [³H]R1881 binding in the presence of hydroxyflutamide (OHF).