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Transactivation by GR in wild-type and cdc37–34 mutant strains. (A) β-Galactosidase activity in wild-type (WT; lanes 1 and 2) and cdc37–34 (lanes 3 and 4) mutant strains containing GR. Cultures were incubated at 25°C (A) or 37°C (B) for 1 h before addition of DOC to 100 nM. Samples in lanes 1 and 3 contained no hormone. Results are the mean of three independent experiments.
